M9 minimal medium (M9)
^^^^^^^^^^^^^^^^^^^^^^
The M9 minimal medium (M9) *in silico* definition consists of all compounds described by `Sigma-Aldrich 
<https://www.sigmaaldrich.com/DE/de/product/sigma/m6030>`__. M9 is a minimal medium commonly used in microbiology and 
was developed in 1988\ :footcite:p:`becardEarlyEventsVesicular1988`. Since then, M9 has been adapted with different 
kinds of additives and used to gather insight into carbon or nitrogen sources\ 
:footcite:p:`hawkeyCuratedCollectionKlebsiella2022`. The medium definition used in this study is based on the provider 
reference\ :footcite:p:`M9Minimalsalze5XPowder` and was further supplemented with MgSO\ :sub:`4`\ \* 7H\ :sub:`2`\ O, 
CaCl\ :sub:`2`\ \* 2H\ :sub:`2`\ O and Glucose\ :footcite:p:`Baeuerle2023_M9_repo,Baeuerle2023_M9`.

.. list-table:: M9 composition
  :name: m9_comp
  :align: center
  :widths: 54 26
  :header-rows: 1
  :class: no-scrollbar-table

  * - name
    - source
  * - Ammonia
    - NH4Cl
  * - Calcium(II) cation [Ca(2+)]
    - CaCl2*2H2O
  * - Chloride [Cl(-)]
    - NaCl, NH4Cl, CaCl2*2H2O
  * - D-Glucose
    - Glucose
  * - Dioxygen [O2]
    - Oxygen
  * - Hydrogen [H(+)]
    - KH2PO4, Na2HPO4
  * - Magnesium(II) cation [Mg(2+)]
    - MgSO4*7H2O
  * - Phosphate [PO4(3-)]
    - KH2PO4, Na2HPO4
  * - Potassium(I) cation [K(+)]
    - KH2PO4
  * - Sodium(I) cation [Na(+)]
    - NaCl, Na2HPO4
  * - Sulfate [SO4(2-)]
    - MgSO4*7H2O
  * - Water [H2O]
    - MgSO4*7H2O, CaCl2*2H2O

.. footbibliography::