Multi-Purpose Artificial Urine (MP-AU)

The Mulit-Purpose Artificial Urine is composed of compounds contained within urine as described in ‘A New Artificial Urine Protocol to Better Imitate Human Urine’[1]. Pan, Altenried and Scheibler et al.[2] used MP-AU with Pseudomonas aeruginosa under aerobic conditions. Thus, oxygen is added to the definition.

Table 7 MP-AU composition

BiGG ID	Compound name
na1	Sodium [NaCl]
cl	Chloride [NaCl]
so4	Sulfate [Na2SO4]
urate	Urate [Uric acid (C5H4N4O3)]
cit	Citrate [Sodium citrate (Na3C6H5O7.2H2O)]
crtn	Creatinine [C4H7N3O]
urea	Urea [CH4N2O]
k	Potassium [KCl]
ca2	Calcium [CaCl2]
nh4	Ammonium [NH4Cl]
oxa	Oxalate [Potassium Oxalate (K2C2O4.H2O)]
mg2	Magnesium [MgSO4.7H2O]
h2	Hydrogen [NaH2PO4.2H2O]
pi	Phosphate [NaH2PO4.2H2O]
h	H+ [Na2HPO4.2H2O]
h2o	H2O
o2	O2 [Oxygen]

[1]

Neslihan Sarigul, Filiz Korkmaz, and İlhan Kurultak. A new artificial urine protocol to better imitate human urine. Scientific Reports, 9(1):20159, Dec 2019. URL: https://doi.org/10.1038/s41598-019-56693-4, doi:10.1038/s41598-019-56693-4.

[2]

Fei Pan, Stefanie Altenried, Subas Scheibler, Alexandre H.C. Anthis, and Qun Ren. Specific capture of pseudomonas aeruginosa for rapid detection of antimicrobial resistance in urinary tract infections. Biosensors and Bioelectronics, 222:114962, 2023. URL: https://www.sciencedirect.com/science/article/pii/S0956566322010028, doi:https://doi.org/10.1016/j.bios.2022.114962.