Basal Medium as described in Swaney et al. 2023 (BMS23)

This Basal Medium (BMS23) defintion contains all components as described in ‘Sweat and Sebum Preferences of the Human Skin Microbiota’[1] (Material & Methods). The M9 components where derived from Sigma-Aldrich.

Table 1 BMS23 composition

name	source
3-[(2R)-2,4-Dihydroxy-3,3-dimethylbutanamido]propanoate [Pantothenate]	Calcium pantothenate
Ammonia	From M9: NH4Cl
Biotin	Biotin
Calcium(II) cation [Ca(2+)]	Calcium Chloride anhydrous, Calcium pantothenate
Chloride [Cl(-)]	Cobalt Chloride hexahydrate, Calcium Chloride anhydrous, Thiamine HCl; From M9: NaCl, NH4Cl
Cobalt(II) cation [Co(2+)]	Cobalt Chloride hexahydrate
D-Glucose	Glucose
Dioxygen [O2]	Oxygen
Hydrogen [H(+)]	Thiamine HCl, From M9: KH2PO4, Na2HPO4
L-Arginine	L-Arginine
L-Proline	L-Proline
Magnesium(II) cation [Mg(2+)]	Magnesium Sulfate heptahydrate
Phosphate [PO4(3-)]	From M9: KH2PO4, Na2HPO4
Potassium(I) cation [K(+)]	From M9: KH2PO4
Pyridine-3-carboxylate [Niacin]	Nicotinic acid
Sodium(I) cation [Na(+)]	From M9: NaCl, Na2HPO4
Sulfate [SO4(2-)]	Magnesium Sulfate heptahydrate
Thiamine	Thiamine HCl
Water [H2O]	Cobalt Chloride hexahydrate, Magnesium Sulfate heptahydrate

[1]

Mary Hannah Swaney, Amanda Nelsen, Shelby Sandstrom, and Lindsay R. Kalan. Sweat and sebum preferences of the human skin microbiota. Microbiology Spectrum, 11(1):e04180–22, 2023. URL: https://journals.asm.org/doi/abs/10.1128/spectrum.04180-22, arXiv:https://journals.asm.org/doi/pdf/10.1128/spectrum.04180-22, doi:10.1128/spectrum.04180-22.