Roswell Park Memorial Institute 1640 Medium (RPMI)

RPMI is a medium used in cell culture and was developed in 1966 at the Roswell Park Memorial Institute by Moore et al.[1]. Its exact formulation is known and available via ThermoFisher Scientific[2].

Table 14 RPMI composition

name

source

3-[(2R)-2,4-Dihydroxy-3,3-dimethylbutanamido]propanoate [Pantothenate]

D-Calcium pantothenate

4,5-Bis(hydroxymethyl)-2-methylpyridin-3-ol [Pyridoxine]

Pyridoxine hydrochloride

4-Aminobenzoic acid

Para-Aminobenzoic Acid

Biotin

Biotin

Calcium(II) cation [Ca(2+)]

D-Calcium pantothenate, Calcium nitrate (Ca(NO3)2 4H2O)

Carbon dioxide [CO2]

Carbon dioxide

Hydrogencarbonate

Bicarbonate (NaHCO3)

Chloride [Cl(-)]

L-Cystine 2HCl, L-Lysine hydrochloride, Choline chloride, Pyridoxine hydrochloride, Thiamine hydrochloride, Potassium Chloride (KCl), Sodium Chloride (NaCl)

Choline

Choline chloride

Cob(I)alamin

Vitamin B12

Cob(II)alamin

Vitamin B12

D-Glucose

D-Glucose (Dextrose)

Dioxygen [O2]

Oxygen

Glycine

Glycine

Hydrogen [H(+)]

L-Cystine 2HCl, L-Lysine hydrochloride, L-Tyrosine disodium salt dihydrate, Pyridoxine hydrochloride, Thiamine hydrochloride

L-Cystine

L-Cystine 2HCl

L-Arginine

L-Arginine

L-Asparagine

L-Asparagine

L-Aspartate

L-Aspartic acid

L-Glutamate

L-Glutamic Acid

L-Glutamine

L-Glutamine

L-Histidine

L-Histidine

L-Isoleucine

L-Isoleucine

L-Leucine

L-Leucine

L-Lysine

L-Lysine hydrochloride

L-Methionine

L-Methionine

L-Phenylalanine

L-Phenylalanine

L-Proline

L-Proline

L-Serine

L-Serine

L-Threonine

L-Threonine

L-Tryptophan

L-Tryptophan

L-Tyrosine

L-Tyrosine disodium salt dihydrate

L-Valine

L-Valine

L-gamma-Glutamyl-L-cysteinylglycine [Glutathione]

Glutathione (reduced)

Magnesium(II) cation [Mg(2+)]

Magnesium Sulfate (MgSO4) (anhyd.)

Myo-inositol

i-Inositol

N-(4-{[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino}benzoyl)-L-glutamic acid [Folate]

Folic Acid

Niacinamide

Niacinamide

Nitrate [NO3(-)]

Calcium nitrate (Ca(NO3)2 4H2O)

Phosphate [PO4(3-)]

Sodium Phosphate dibasic (Na2HPO4) anhydrous

Potassium(I) cation [K(+)]

Potassium Chloride (KCl)

Riboflavin

Riboflavin

Sodium(I) cation [Na(+)]

L-Tyrosine disodium salt dihydrate, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous

Sulfate [SO4(2-)]

Magnesium Sulfate (MgSO4) (anhyd.)

Thiamine

Thiamine hydrochloride

Vitamin B12

Vitamin B12

Water [H2O]

Calcium nitrate (Ca(NO3)2 4H2O)

trans-4-Hydroxy-L-proline

L-Hydroxyproline