Multi-Purpose Artificial Urine (MP-AU)

The Mulit-Purpose Artificial Urine is composed of compounds contained within urine as described in ‘A New Artificial Urine Protocol to Better Imitate Human Urine’[1]. Pan, Altenried and Scheibler et al. [2] used MP-AU with Pseudomonas aeruginosa under aerobic conditions. Thus, oxygen is added to the definition.

Table 9 MP-AU composition

name	source
2-Oxidopropane-1,2,3-tricarboxylate [Citrate]	Na3C6H5O7.2H2O
Ammonia	NH4Cl
Calcium(II) cation [Ca(2+)]	CaCl2
Chloride [Cl(-)]	KCl, NaCl, CaCl2, NH4Cl
Creatinine	C4H7N3O
Dioxygen [O2]	Oxygen
Hydrogen [H(+)]	NaH2PO4.2H2O, Na2HPO4.2H2O
Magnesium(II) cation [Mg(2+)]	MgSO4.7H2O
Oxalate	K2C2O4.H2O
Phosphate [PO4(3-)]	NaH2PO4.2H2O, Na2HPO4.2H2O
Potassium(I) cation [K(+)]	KCl, K2C2O4.H2O
Sodium(I) cation [Na(+)]	Na2SO4, Na3C6H5O7.2H2O, NaCl, NaH2PO4.2H2O, Na2HPO4.2H2O
Sulfate [SO4(2-)]	Na2SO4, MgSO4.7H2O
Urate	C5H4N4O3
Urea	CH4N2O
Water [H2O]	Na3C6H5O7.2H2O, K2C2O4.H2O, MgSO4.7H2O, NaH2PO4.2H2O, Na2HPO4.2H2O

[1]

Neslihan Sarigul, Filiz Korkmaz, and İlhan Kurultak. A new artificial urine protocol to better imitate human urine. Scientific Reports, 9(1):20159, Dec 2019. URL: https://doi.org/10.1038/s41598-019-56693-4, doi:10.1038/s41598-019-56693-4.

[2]

Fei Pan, Stefanie Altenried, Subas Scheibler, Alexandre H.C. Anthis, and Qun Ren. Specific capture of pseudomonas aeruginosa for rapid detection of antimicrobial resistance in urinary tract infections. Biosensors and Bioelectronics, 222:114962, 2023. URL: https://www.sciencedirect.com/science/article/pii/S0956566322010028, doi:https://doi.org/10.1016/j.bios.2022.114962.