Synthetic Nasal Medium Version 3 (SNM3)

The Synthetic Nasal Medium x3 (SNM3) definition contains all components as described in ‘Nutrient Limitation Governs Staphylococcus aureus Metabolism and Niche Adaptation in the Human Nose’[1].

Table 11 SNM3 composition

name	source
(2E)-But-2-enedioate [Fumarate]	Fumaric acid
2,2-Bipyridine	2,2-Bipyridine
2-Oxidopropane-1,2,3-tricarboxylate [Citrate]	Citric acid
3-[(2R)-2,4-Dihydroxy-3,3-dimethylbutanamido]propanoate [Pantothenate]	Ca-D-Panthothenic acid
4-(Azaniumylmethyl)-3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-1-ium [Pyridoxamine]	Pyridoxamine-2HCl
4-Aminobenzoic acid	p-Aminobenzoic acid
Biotin	Biotin
Calcium(II) cation [Ca(2+)]	Ca-D-Panthothenic acid
Carbon dioxide [CO2]	Carbon dioxide
Chloride [Cl(-)]	Chloride, NaCl, KCl, ZnCl2, MnCl2, CoCl2, CuCl2, NiCl2, Pyridoxamine-2HCl, Thiamine-dichoride
Cob(I)alamin	Cyanocobalamine
Cob(II)alamin	Cyanocobalamine
Cobalt(II) cation [Co(2+)]	CoCl2
Copper(II) cation [Cu(2+)]	CuCl2
D-Glucose	Glucose
D-Ornithine	Ornithine
Dioxygen [O2]	Oxygen
Glycine	Glycine
Hydrogen [H(+)]	Pyridoxamine-2HCl, Thiamine-dichoride
L-Alanine	Alanine
L-Arginine	Arginine
L-Cysteine	Cysteine
L-Glutamate	Glutamic acid
L-Histidine	Histidine
L-Leucine	Leucin
L-Lysine	Lysine
L-Ornithine	Ornithine
L-Phenylalanine	Phenylalanine
L-Proline	Proline
L-Serine	Serine
L-Threonine	Threonine
L-Tryptophan	Tryptophane
L-Valine	Valine
Magnesium(II) cation [Mg(2+)]	Magnesium, MgSO4
Maleate	Maleic acid
Manganese(II) cation [Mn(2+)]	MnCl2
Nickel(II) cation [Ni(2+)]	NiCl2
Phosphate [PO4(3-)]	Phosphate, Sodium phosphate buffer (pH7.2)
Potassium(I) cation [K(+)]	Potassium, KCl
Pyridine-3-carboxylate [Niacin]	Nicotinic acid
Pyruvate [Pyruvic acid]	Pyruvic acid
Riboflavin	Riboflavin
Sodium(I) cation [Na(+)]	Sodium, Sodium phosphate buffer (pH7.2), NaCl, MoNa2O4
Succinate [Succinic acid]	Succinic acid
Sulfate [SO4(2-)]	Sulphate
Tetraoxidomolybdate(VI) [Molybdate]	MoNa2O4
Thiamine	Thiamine-dichoride
Urea	Urea
Vitamin B12	Cyanocobalamine
Water [H2O]	Water
Zinc(II) cation [Zn(2+)]	ZnCl2

[1]

Bernhard Krismer, Manuel Liebeke, Daniela Janek, Mulugeta Nega, Maren Rautenberg, Gabriele Hornig, Clemens Unger, Christopher Weidenmaier, Michael Lalk, and Andreas Peschel. Nutrient limitation governs staphylococcus aureus metabolism and niche adaptation in the human nose. PLOS Pathogens, 10(1):1–17, 01 2014. URL: https://doi.org/10.1371/journal.ppat.1003862, doi:10.1371/journal.ppat.1003862.