Corynebacterium glutamicum optimized growth medium (CGXII)

CGXII is a medium developed to optimize growth of Corynebacterium glutamicum. Since C. glutamicum is used widely in biotechnology applications, it was of interest to optimize a medium just for this species[1].

Table 5 CGXII composition

name	source
3,4-Dihydroxybenzoate	Protocatechuic acid
Ammonia	(NH4)2SO4
Biotin	Biotin
Calcium(II) cation [Ca(2+)]	CaCl2.2H2O
Chloride [Cl(-)]	CaCl2.2H2O, NiCl2.6H2O
Copper(II) cation [Cu(2+)]	CuSO4.5H2O
D-Glucose	D-Glucose
Dioxygen [O2]	Oxygen
Hydrogen [H(+)]	K2HPO4, KH2PO4
Iron(II) cation [Fe(2+)]	FeSO4.7H2O
Iron(III) cation [Fe(3+)]	FeSO4.7H2O
Magnesium(II) cation [Mg(2+)]	MgSO4.7H2O
Manganese(II) cation [Mn(2+)]	MnSO4.H2O
Nickel(II) cation [Ni(2+)]	NiCl2.6H2O
Phosphate [PO4(3-)]	K2HPO4, KH2PO4
Potassium(I) cation [K(+)]	K2HPO4, KH2PO4
Sodium(I) cation [Na(+)]	NaOH
Sulfate [SO4(2-)]	(NH4)2SO4, MgSO4.7H2O, FeSO4.7H2O, MnSO4.H2O, CuSO4.5H2O, ZnSO4.7H2O
Urea	Urea
Water [H2O]	CaCl2.2H2O, MgSO4.7H2O, FeSO4.7H2O, MnSO4.H2O, NiCl2.6H2O, CuSO4.5H2O, ZnSO4.7H2O, NaOH
Zinc(II) cation [Zn(2+)]	ZnSO4.7H2O
Hydroxide	NaOH

[1]

Simon Unthan, Alexander Grünberger, Jan van Ooyen, Jochem Gätgens, Johanna Heinrich, Nicole Paczia, Wolfgang Wiechert, Dietrich Kohlheyer, and Stephan Noack. Beyond growth rate 0.6: What drives Corynebacterium glutamicum to higher growth rates in defined medium. Biotechnology and Bioengineering, 111(2):359–371, 2014. doi:10.1002/bit.25103.