Chemically Defined Medium as described in Wegkamp et al. 2010 (CDMW10)

The CDMW10 medium is a chemically defined medium that is described by Wegkamp et al.[1]. The following in silico definition contains all components as described in ‘Development of a minimal growth medium for Lactobacillus plantarum’[1] (Table 1).

Table 6 CDMW10 composition

name	source
2-Oxidopropane-1,2,3-tricarboxylate [Citrate]	Ammonium citrate
3-[(2R)-2,4-Dihydroxy-3,3-dimethylbutanamido]propanoate [Pantothenate]	Panthothenic acid
4,5-Bis(hydroxymethyl)-2-methylpyridin-3-ol [Pyridoxine]	Pyridoxine
4-(Azaniumylmethyl)-3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-1-ium [Pyridoxamine]	Pyridoxamine
4-Aminobenzoic acid	Para-aminobenzoic acid
5-(1,2-Dithiolan-3-yl)pentanoate [Lipoate]	6,8-thiotic acid
Acetate [Acetic acid]	Sodium acetate
Adenine	Adenine
Ammonia	Ammonium citrate
Biotin	Biotin
Calcium(II) cation [Ca(2+)]	CaCl2
Chloride [Cl(-)]	MgCl2
Cobalt(II) cation [Co(2+)]	CoSO4
Copper(II) cation [Cu(2+)]	CuSO4
D-Alanine	Alanine
D-Glucose	Glucose
D-Serine	Serine
Glycine	Glycine
Hydrogen [H(+)]	Water,(NH4)6Mo7O24
Inosine	Inosine
Iron(II) cation [Fe(2+)]	FeCl2
Iron(III) cation [Fe(3+)]	FeCl3
L-Alanine	Alanine
L-Arginine	Arginine
L-Ascorbate [Ascorbic acid]	Ascorbic acid
L-Aspartate	Aspartic acid
L-Cysteine	Cysteine
L-Glutamate	Glutamate
L-Histidine	Histidine
L-Isoleucine	Isoleucine
L-Leucine	Leucine
L-Lysine	Lysine
L-Methionine	Methionine
L-Phenylalanine	Phenylalanine
L-Proline	Proline
L-Serine	Serine
L-Threonine	Threonine
L-Tryptophan	Tryptophan
L-Tyrosine	Tyrosine
L-Valine	Valine
Magnesium(II) cation [Mg(2+)]	MgCl2
Manganese(II) cation [Mn(2+)]	MnCl2
Orotate [Orotic acid]	Orotic acid
Phosphate [PO4(3-)]	Phosphate
Potassium(I) cation [K(+)]	From buffer
Pyridine-3-carboxylate [Niacin]	Nicotinic acid
Riboflavin	Riboflavin
Sodium(I) cation [Na(+)]	Sodium acetate
Sulfate [SO4(2-)]	ZnSO4
Tetraoxidomolybdate(VI) [Molybdate]	(NH4)6Mo7O24
Thiamine	Thiamine
Thymidine	Thymidine
Uracil	Uracil
Vitamin B12	Vitamin B12
Water [H2O]	Water,(NH4)6Mo7O24
Zinc(II) cation [Zn(2+)]	ZnSO4
2-amino-1,9-dihydro-6H-purin-6-one [Guanine]	Guanine
3,7-Dihydro-1H-purine-2,6-dione [Xanthine]	Xanthine

[1] (1,2)

A. Wegkamp, B. Teusink, W. M. de Vos, and E. J. Smid. Development of a minimal growth medium for Lactobacillus plantarum. Letters in Applied Microbiology, 50(1):57–64, January 2010. doi:10.1111/j.1472-765X.2009.02752.x.