Plantarum Minimal Medium for L. plantarum WCFS1 (PMM5, true minimal medium)

PMM5 is a true minimal medium for Lactiplantibacillus (former: Lactobacillus) plantarum WCFS1 first described by Wegkamp et al.[1]. The following in silico definition contains all components as described in ‘Development of a minimal growth medium for Lactobacillus plantarum’[1] (Table 1).

Table 12 PMM5 composition

name	source
3-[(2R)-2,4-Dihydroxy-3,3-dimethylbutanamido]propanoate [Pantothenate]	Panthothenic acid
Acetate [Acetic acid]	Sodium acetate
Chloride [Cl(-)]	MgCl2
D-Glucose	Glucose
Hydrogen [H(+)]	Water
L-Arginine	Arginine
L-Ascorbate [Ascorbic acid]	Ascorbic acid
L-Glutamate	Glutamate
L-Isoleucine	Isoleucine
L-Leucine	Leucine
L-Methionine	Methionine
L-Phenylalanine	Phenylalanine
L-Threonine	Threonine
L-Tryptophan	Tryptophan
L-Tyrosine	Tyrosine
L-Valine	Valine
Magnesium(II) cation [Mg(2+)]	MgCl2
Manganese(II) cation [Mn(2+)]	MnCl2
Phosphate [PO4(3-)]	Phosphate
Potassium(I) cation [K(+)]	From buffer
Pyridine-3-carboxylate [Niacin]	Nicotinic acid
Riboflavin	Riboflavin
Sodium(I) cation [Na(+)]	Sodium acetate
Water [H2O]	Water

[1] (1,2)

A. Wegkamp, B. Teusink, W. M. de Vos, and E. J. Smid. Development of a minimal growth medium for Lactobacillus plantarum. Letters in Applied Microbiology, 50(1):57–64, January 2010. doi:10.1111/j.1472-765X.2009.02752.x.